DEMO
Pumping Demonstration

To test the pump an inlet container filled with water was added below the height of the pump and the inlet tube of the pump was submerged in the water. An empty outlet container was placed above the height of the pump and the outlet tube of the pump was inserted into the outlet container. To actuate the pump, the plunger of the syringe was retracted, sucking in air to the barrel and deflating the actuator pockets. This created a vacuum in the pump that pulled water from the inlet container through the inlet valve into the pump. Water was pulled into the pump despite not priming the pump with water beforehand and having air in the line. The plunger was then advanced, pushing air into the pockets of the actuator, inflating them. The inflated pockets displaced the fluid in the chamber analogous to muscle contraction in the heart, pumping fluid out of the pump through the outlet valve into the outlet container. The one-way inlet and outlet valves prevent fluid from moving from the outlet to the inlet, directing flow only from the inlet to the outlet.
Inside Chamber

The video shows the inflation of the soft pneumatically actuated pump resulting in pumping/ejection of the fluid from the chamber.
Functional Evaluation
Normal ejection fraction of the left ventricle of the human heart has been reported to be greater than 50 percent (Paulus, W.J. and van Ballegoij, J.J., 2010). To quantify the function of the fluid pump, ejection fraction of the pump was found by measuring the volume of fluid contained in the chamber in two actuator modes, when relaxed and then inflated and when deflated and then inflated. Three trials were performed for each actuator mode. The ejection fraction for each trial and actuator mode was calculated and the average was found for each mode as seen in the table below. The measurements show that the soft polymer pneumatically actuated pump was able to produce an ejection fraction comparable to a normally functioning human heart with the average standard mode and maximum mode ejection fractions of 64.9% and 69.5% respectively.

Reference
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Paulus, W.J. and van Ballegoij, J.J., 2010. Treatment of heart failure with normal ejection
fraction: an inconvenient truth!. Journal of the American College of Cardiology, 55(6), pp.526-537.